|
KMID : 0364820150510020177
|
|
Korean Journal of Microbiology
2015 Volume.51 No. 2 p.177 ~ p.180
|
|
|
|
|
Jung Taeck-Kyung
Song Hong-Gyu
|
|
|
Abstract
|
|
|
|
For prevention of atrophic rhinitis of swine by Bordetella bronchiseptica and fowl typhoid by Salmonella gallinarum, bacterial strains showing antimicrobial activity against those pathogenic bacteria were isolated from various samples collected at animal farms. Among 372 bacterial isolates strain TK3 showed the highest antibacterial activity against both pathogens, and was identified as Bacillus amyloiiquefaciens by 16S rRNA gene sequence analysis. B. amyloliquefaciensTK3 could inhibit growth of both pathogens by secretion of antibacterial compounds such as siderophore, rhamnolipid and antimicrobial peptide. Production radius of siderophore on Chrome azurol S agar plate by strain TK3 was 0.53 cm after 14 days of incubation, and concentration of siderophore in King’s B medium was 1.06 mmol/ml. It also secreted 82.4 mg/L of rhamnolipid, and antimicrobial peptide that completely inhibited growth of both pathogens at concentration of 30 μl/ml in LB medium.
|
|
|
KEYWORD
|
|
|
Bacillus amyloliquefaciens, Bordetella bronchiseptica, Salmonella gallinarum, antibacterial activity
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|